Talent qPCR PreMix (SYBR Green) Featured Image

Talenta qPCR PreMix (SYBR Green)

Ho hanyetsa hantle tšitiso ea ho se hloeke le ho etsa li-template tse rarahaneng ka potlako.

Sehlahisoa sena ke reagent e khethehileng bakeng sa Real-Time PCR e sebelisang SYBR Green I chimeric fluorescence. The 2 × Talent qPCR PreMix e amohela anti Taq DNA polymerase, 'me sistimi e ikhethileng ea qPCR Buffer e ka netefatsa sehlahisoa ho etsa tšebetso ea qPCR e bonolo le e potlakileng ho lisebelisoa tsohle tsa Real-Time PCR. Nako ea karabelo ea qPCR e ka khutsufatsoa ka 50%. Ntle le moo, tlatsetso ea karolo ea H-Competitor le karolo ea EP ho Buffer e etsa hore sehlahisoa se be le mehlala e mengata ea bokahohleng. E na le ts'ebeliso e ntle haholo ho li-template tse nang le meaho e rarahaneng e tsoetseng pele, litempele tse nang le masala a mangata a PCR inhibitor, amplification e telele ea sekhechana, jj.
Sehlahisoa se boetse se na le litšobotsi tsa sekhahla se phahameng sa kholiso, bophahamo bo phahameng ba kholiso, mefuta e mengata e tšepahalang, jj.

Katse. Che	Paka Size
4992882	20 ×l × 125 rxn
4992775	20 ×l × 500 rxn
4992866	20 ×l × 5000 rxn

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Likaroloana

Antib Lesole le fetohang haholo la 'mele le fetotsoeng ka polymerase le ka khutsufatsa nako ka halofo.
■ H-Competitor e qothisana lehlokoa le hydrogen bond, e netefatsang ts'ebetso e phahameng ea ho matlafatsa litempele tse nang le litaba tse phahameng tsa GC, sebopeho se rarahaneng sa bobeli le litempele tse telele.
■ Karolo ea EP e tsitsisa sistimi ea PCR, e sireletsa ka nepo tšebetso ea enzyme mme e hanela ho kena-kenana le li-inhibitors tse fapaneng.
■ Tube e se nang mmala e bonaletsang e nka sebaka sa tube e sootho, e le hore chelete e setseng e bonahale.

Likopo

E loketse tlhahlobo ea polelo, phumano ea acid ea nucleic le mefuta e meng ea liteko ho lisebelisoa tse fapaneng tsa nako ea PCR ka mokhoa oa SYBR Green, haholoholo bakeng sa GC e phahameng, sebopeho se rarahaneng sa bobeli, masala a phahameng a litšila le palo e telele ea template.

Lihlahisoa tsohle li ka fetoloa bakeng sa ODM / OEM. Bakeng sa lintlha,ka kopo tlanya Customized Service (ODM / OEM)

E fetileng: FastFire qPCR PreMix (Phuputso)

E 'ngoe: Kit ea Ts'ebetso ea HRM (EvaGreen)

Experimental Example

Lefu la motho la UH11 (GC: 73.4%) le lekantsoe ke TIANGEN Talent qPCR PreMix (ka ho le letšehali) le sehlahisoa se loketseng ho tsoa ho Mofani oa A (bohareng) le B (ka ho le letona). Liphetho li bonts'a hore ha li bapisoa le barekisi ba bang, Talent qPCR PreMix e na le li-curve tse hlakileng tsa li-gradients tsa mahloriso tse 5, boleng bo tlase ba Ct, boleng bo phahameng ba fluorescence, le tlhoro e le 'ngoe e qhibilihisang, e bonts'ang Talent qPCR PreMix e na le bokhoni bo botle ba ho ikamahanya le maemo le matlafatso ho litempele tse phahameng tsa GC.

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