Kit ea Ts'ebetso ea HRM (EvaGreen)

Likaroloana

■ Qeto e phahameng: Amohela lidae tse tlatsitsoeng tsa EvaGreen, tse nang le tharollo e phahameng ea leqhubu le qhibilihisang le maemong a phahameng mme e ka khetholla phapano ea motheo o le mong.
■ Ho khetheha ka mokhoa o phahameng: Sebelisa DNA polymerase e chesang ea anti-antibody ho fokotsa matlafatso a sa amaneng le ho ntlafatsa maemo.
■ botsitso bo phahameng: Sistimi ea Buffer e ntlafalitseng ka hloko e eketsa botsitso ba mothinya o qhibilihisang le ho ntlafatsa ts'epahalo ea liphetho.
■ Khalemelo ea ROX: Dae ea ROX e kentsoe ka thoko, e leng bonolo ho e sebelisa ebile e na le liphetho tse nepahetseng.

Ga tlhaloso

Mofuta: Antibody e fetotsoeng Taq DNA polymerase, EvaGreen.
Likopo: Ho tsebahala ka SNP; Tlhatlhobo e sa tsejoeng ea SNP; Ho sekaseka liphatsa tsa lefutso tse sa tsejoeng; Tlhahlobo ea Methylation PCR.

Lihlahisoa tsohle li ka fetoloa bakeng sa ODM / OEM. Bakeng sa lintlha,ka kopo tlanya Customized Service (ODM / OEM)

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Genomic DNA e ntšitsoe ho 100 μl ea sampole ea mali a motho e sebelisa TIANamp Blood DNA Kit. 50 ng DNA e ile ea laeloa ka nako ea 'nete ea phumano ea PCR. Ho latela laeborari ea NCBI SNP, SNP e tsebahalang ea FEN1 gene (RS 174538) e khethiloe le ho fumanoa ka ho sebelisa Roche LightCycle480. Liphetho ke tse latelang:

Liphetho li bonts'a hore HRM Analysis Kit ke mokhoa o nepahetseng oa SNP locus analysis le ho pheta-pheta ho phahama ho qhibilihang ha mofuta o tšoanang, tharollo e hlakileng ea li-genotypes tse fapaneng mme ho se na kahlolo e fosahetseng.

Tlhahisoleseling ea SNP: ……. CGGAAGAACACGTCG [A / G] CAGGAGCAGGCGCCT …… (Allele Frequency: A = 0.314, G = 0.686)

Tlhahiso-pele: Bakeng sa sekhechana sa bp sa 108 (FEN1_F: CCTCAACGCTCTCACCATTTTG; FEN1_R: GGCACTTCCTTTTCCGGTTGTG)