■ Lipontšo tsa mebala li eketsoa ho li-reagents ho qoba liphoso tsa tlatsetso ea sampole kapa ho se be teng ka lebaka la palo e kholo ea mehlala.
■ Boleng bo phahameng ba khanya, kutlo e phahameng le ho khetheha ho hoholo.
■ Kamora hore e lemohuoe ka thata, sesupo se ke ke sa ama boleng ba fluorescence le sephetho sa bongata ba dae.
■ E loketse mefuta e fapaneng ea liletsa tse tloaelehileng tsa nako ea sebele ea PCR (Roche, Bio-rad, ABI, jj.).
E loketse mefuta e fapaneng ea liteko tse kang tlhahlobo ea polelo le tlhahlobo ea acidic ea nucleic ka mokhoa oa SYBR Green ho lisebelisoa tse fapaneng tsa nako ea PCR. E loketse haholo-holo liteko tse phahameng tsa tlhahiso le palo e kholo ea mehlala le ts'ebetso e rarahaneng ea ho jarolla mehlala.
Lihlahisoa tsohle li ka fetoloa bakeng sa ODM / OEM. Bakeng sa lintlha,ka kopo tlanya Customized Service (ODM / OEM)
Setšoantšo. 1. Lirafo tsa li-curve tsa amplifi cation le li-curve tse qhibilihisang tse fumanoeng ka ho latellana ha liphatsa tsa lefutso tsa HS1 tsa motho li sebelisa TIANGEN SuperReal PreMix Plus (SYBR Green) (A) le SuperReal PreMix Colour (SYBR Green) (B). Template e hlapollotsoe bakeng sa li-gradients tse 5 tsa mahloriso. Litekanyetso tsa Ct (C) tse fumanoeng ka papiso li bonts'a hore ho matlafatsoa ha lisebelisoa tse peli ho na le kutloisiso, boits'oaro le botsitso. Teko e tsamaisitsoe ho Roche LightCycler480. | |
Setšoantšo sa 2. Li-graph tsa kholiso ea molumo le mothinya o qhibilihisang o fumanoeng ka ho latellana ha liphatsa tsa lefutso tsa HS5 tsa motho ho sebelisa TIANGEN SuperReal PreMix Colour (SYBR Green) le sehlahisoa se loketseng ho tsoa ho Mofani oa T (A: TIANGEN, B: Mofani oa thepa. mme o bapise boleng ba Ct (C). Sephetho se bonts'a hore SuperReal PreMix Colour (SYBR Green) e na le kutlo e phahameng, ho khetheha le botsitso. Teko e sebelisitsoe ho Bio-Rad CFX96. |
Ho tloha ha e theoa, fektheri ea rona e ntse e nts'etsapele lihlahisoa tsa maemo a pele ka ho latela molao-motheo
ea boleng pele. Lihlahisoa tsa rona li fumane botumo bo botle indastering ebile li na le boleng bo phahameng har'a bareki ba bacha le ba khale ..