■ Tšireletso e sebetsang: Sireletsa RNA maling a hloekileng hore a se ke a senyeha.
■ E bonolo: Ts'ebetso e nka mehato e 2 feela mme e loketse poloko ea lisampole tse ngata tsa mali.
■ Ts'ebelisano e phethahetseng: E lumellana le thepa ea TIANGEN e nang le thepa ea Silicon Matrix Membrane Purification Kits ho ntša mali a RNA kapa DNA ho netefatsa tlhahiso ea ho ntša le ho hloeka.
Lihlahisoa tsohle li ka fetoloa bakeng sa ODM / OEM. Bakeng sa lintlha,ka kopo tlanya Customized Service (ODM / OEM)
Lintho tse bonahalang: 100 μl mali a hloekileng a mouse Mokhoa: RNA e felletseng ea mali a macha a macha (a bolokiloeng ho RNALock Reagent) a neng a bolokiloe ho 15-25 ℃, 4-8 ℃, -20 / -70 ℃ a ne a arotsoe a sebelisa RNAprep Pure Blood Kit (4992238) kamora protocol ea RNALock . Liphetho: Ka kopo bona setšoantšo se kaholimo sa agarose gel electrophoresis. 4-6 μl ea 50 μl eluates e ile ea laeloa moleng o mong le o mong. M: TIANGEN DNA Letšoao la III; Lane 1-2 (taolo e nepahetseng): RNA e hloekisitsoeng maling a hloekileng; Lane 3-4: RNA e hloekisitsoe liseleng tsa mali tse bolokiloeng ka 15-25 ℃ matsatsi a 2. Lane 5-6: RNA e hloekisitsoe liseleng tsa mali tse bolokiloeng ho 4-8 ℃ beke e le 'ngoe. Lane 7-8: RNA e hloekisitsoe liseleng tsa mali tse bolokiloeng ho -20 ℃ kapa -70 ℃ halofo ea selemo. Electrophoresis e tsamaisitsoe ka 6 V / cm bakeng sa 30 min ho 1% agarose. |
Ho tloha ha e theoa, fektheri ea rona e ntse e nts'etsapele lihlahisoa tsa maemo a pele ka ho latela molao-motheo
ea boleng pele. Lihlahisoa tsa rona li fumane botumo bo botle indastering ebile li na le boleng bo phahameng har'a bareki ba bacha le ba khale ..