Matla a khoheli a Litholoana tsa Genetic DNA Kit

Ho itšehla thajana le ho hloekisa DNA ea boleng bo holimo ea "genomic" ho tsoa lisele tsa liphoofolo.

Thepa e amohela lifaha tsa makenete tse nang le tšebetso e ikhethileng ea karohano le sistimi e ikhethileng ea buffer ho arola le ho hloekisa DNA ea boleng bo holimo ea genomic ho tsoa mehlala ea lisele tsa liphoofolo. Lifaha tse ikhethileng tse iketlileng li na le kamano e matla ea acid ea nucleic tlasa maemo a itseng. Ha maemo a fetoha, lifaha tsa makenete li tla ntša acidic e entsoeng ka adsorbed, ka hona e fihlella sepheo sa karohano e potlakileng le tlhoekiso ea acid ea nucleic. Ts'ebetso eohle e bolokehile ebile e bonolo. Ka likhechana tse kholo tsa DNA tsa genomic, bohloeki bo phahameng le boleng bo tšepahalang, mokhoa ona o loketse haholo-holo ho qhekella litsing tsa mesebetsi e phahameng.

Katse. Che Paka Size
4992404 50 li-preps
4992405 200 pele
4992864 1000 pele

Product Qaqileng

Mohlala oa Teko

LBH

Lihlahisoa tsa Product

Likaroloana

■ E bonolo ebile e potlakile: Direct lysis ntle le poloko, le ultrapure genomic DNA e ka fumaneha nakong ea hora e le 'ngoe.
■ Phallo e phahameng: E ​​ka kopanngoa le lisebelisoa tse iketselitsoeng tsa mokhoa oa lipeipi le mokhoa oa matla a khoheli bakeng sa liteko tse phahameng tsa ho qhekella.
■ Sireletsehile ebile ha e na chefo: Ha ho hlokahale li-reagents tse kang phenol / chloroform.
■ Bohloeki bo phahameng: DNA e fumanoeng e na le bohloeki bo phahameng 'me e ka sebelisoa ka kotloloho ha ho fumanoa li-chip, tatellano e phahameng ea liteko le liteko tse ling.

Likopo

DNA e hloekisitsoeng e ka sebelisoa litekong tse fapaneng tse tloaelehileng tse kang ts'ebetso ea enzyme, PCR, kaho ea laeborari, hybridization e ka boroa, chip hybridization, tatellano e phahameng ea ts'ebetso, jj.

Lihlahisoa tsohle li ka fetoloa bakeng sa ODM / OEM. Bakeng sa lintlha,ka kopo tlanya Customized Service (ODM / OEM)


  • E fetileng:
  • E 'ngoe:

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    Experimental Example Sebelisa Kit ea Magnetic Animal Tissue Genomic DNA Kit ho hloekisa DNA ho tsoa liseleng tse fapaneng tsa likhoto, litlhapi, shrimp, shellfish, lisele tse tsoetseng pele, nama ea leoto la letata le mehlala e meng ho latela litaelo. M: Letšoao la TIANGEN D15000.
    Elution volume ke 150 .l. 1-2 elul eluent e ile ea laeloa ho agarose gel electrophoresis.
    Liphetho: TIAGNEN Magnetic Animal Tissue Genomic DNA Kit e na le mefuta e mengata ea ts'ebeliso ea sampole, haholo-holo bakeng sa mehlala e thata joalo ka mohatla oa rat, tlhapi, shrimp le shellfish.
     Experimental Example Sebelisa TIAGNEN Magnetic Animal Tissue Genomic DNA Kit le lisebelisoa tse loketseng tsa Supplier M, O, A ho hloekisa DNA ho tsoa ho 25 mg rat tissue ea pelo ho latela litaelo. M: Letšoao la TIANGEN D15000.
    Elution bophahamo ba modumo: 150 .l. 1 elul eluent e ile ea laeloa ho agarose gel electrophoresis.
    Liphetho: TIAGNEN Magnetic Animal Tissue Genomic DNA Kit e na le chai e ngata haholo.
    P: DNA e nyane kapa ha e eo ho lona.

    A-1 Lisele tsa vaerase kapa vaerase e tlase sampoleng e qalang -E ruisa bongata ba lisele kapa livaerase.

    A-2 Lysis e sa lekanang ea lisampole -Sampole ha e so tsoakane hantle le buffer ea lysis. Ho khothalletsoa ho kopanya ka botlalo ka pulse-vortexing makhetlo a 1-2. —Leseli e sa lekanang ea lisele e bakoang ke ho fokotseha ha tšebetso ea proteinase K. Ho khothalletsoa ho seha lisele ka likotoana tse nyane ebe o eketsa nako ea ho hlapa ho tlosa masalla a lysate.

    A-3 Phatlalatso e sa lekaneng ea DNA. -Ha ho ethanol kapa liperesente tse tlase ho e-na le 100% ea ethanol e ileng ea eketsoa pele lysate e fetisetsoa ho kholomo ea spin.

    A-4 Boleng ba pH ba elution buffer bo tlase haholo. - Fetola pH hore e be lipakeng tsa 8.0-8.3.

    P: DNA ha e sebetse hantle litekong tsa enzymatic reaction reaction.

    E setseng ea ethanol ka eluent.

    -Ho na le masalla a hlatsoang sesupa-tsela PW lehloeng. Ethanol e ka tlosoa ka ho kenya centrifuging spin ea kholomo bakeng sa 3-5 min, ebe e beha mocheso oa kamore kapa 50 ℃ incubator bakeng sa 1-2 min.

    P: Ho senyeha ha DNA

    A-1 Sampole ha e ncha. —Khetholla sampole e ntle ea DNA e le taolo ea ho bona hore na DNA e samporeng e senyehile.

    A-2 Pele ho kalafo e fosahetseng. —E bakoa ke ho sila metsi a naetrojene e feteletseng, ho fumana mongobo hape kapa sampole e ngata haholo.

    Q: U ka etsa pele joang kalafo ea tlhahiso ea gDNA?

    Li-prereatment li lokela ho fapana bakeng sa mehlala e fapaneng. Bakeng sa mehlala ea semela, etsa bonnete ba hore u sila ka botlalo ka naetrojene ea metsi. Bakeng sa disampole tsa liphoofolo, homogenate kapa sila ka botlalo ka naetrojene ea metsi. Bakeng sa mehlala e nang le mabota a sele eo ho leng thata ho e roba, joalo ka baktheria ea G + le tomoso, ho kgothaletswa hore ho sebelisoe lysozyme, lyticase kapa mekhoa ea mochini ho roba mabota a sele.

    P: Phapang ke efe lipakeng tsa lisebelisoa tse tharo tsa tlhahiso ea limela tsa gDNA 4992201/4992202, 4992724/4992725, 4992709/4992710?

    4992201/4992202 Plant Genomic DNA Kit e sebelisa mokhoa o sebelisang kholomo o hlokang chloroform bakeng sa ho ntšoa. E loketse haholo-holo mefuta e fapaneng ea limela, hammoho le phofo e ommeng ea semela. Hi-DNAsecure Plant Kit le eona e thehiloe holima kholomo, empa ha ho hlokahale hore e ntšoe phenol / chloroform, ee boloke e se na chefo. E loketse limela tse nang le polysaccharides e phahameng le polyphenol. 4992709/4992710 DNAquick Plant System e sebelisa mokhoa o sebelisang metsi. Phenol / chloroform ha e hlokehe hape. Tsamaiso ea tlhoekiso e bonolo ebile e potlakile e se na moeli bakeng sa litekanyo tsa sampole e qalang, kahoo basebelisi ba ka fetola chelete ka mokhoa o feto-fetohang ho latela litlhoko tsa liteko. Boholo bo boholo ba likhechana tsa gDNA bo ka fumaneha ka chai e ngata.

    Ke tlhahiso efe e hakantsoeng ea gDNA ho tsoa ho 1 ml sampole ea mali ke TIANamp Blood DNA Kit?

    DNA ea genomic e ntšitsoe mehatong e fapaneng ea lisele tsa motho ka botlalo ke TIANamp Blood DNA Kit. Liphetho li latela. Liphetho li thathamisitsoe e le litšupiso feela, liphetho tsa 'nete tsa ho ntša metsi li latela maemo a mehlala.

    faq

    P: Na 4992207/4992208 le 4992722/4992723 li ka sebelisoa ho ntša mali a DNA?

    Ho ntšoa ha DNA ea mali a mali ho ka etsoa ho sebelisoa li-reagents tse fanoeng ka har'a lisebelisoa tsena tse peli ka ho fetola protocol ho latela taelo e ikhethang ea ho ntšoa ha mali a DNA. Kopi e bonolo ea protocol ea ho ntša mali ea clot ea mali e ka fanoa ha ho kopuoa.

    P: Ha u sebelisa TIANamp Genomic DNA Kit, u ka roba lisele tse ncha hore li fanyehe joang?

    Emisa sampole e ncha ka 1 ml PBS, letsoai le tloaelehileng kapa buffer ea TE. Ka ho feletseng homogenize sampole ke homogenizer le bokella precipitate ho ea tlase ea tube, ke centrifuging. Lahla matla a phahametseng tlhaho, 'me u nchafatse sepakapaka ka 200 μl buffer GA. Tlhoekiso e latelang ea DNA e ka etsoa ho latela taelo.

    P: U ka khetha sehlahisoa sefe bakeng sa ho ntšoa ha DNA ho tsoa liseleng tsa plasma, serum le mokelikeli oa 'mele?

    Bakeng sa tlhoekiso ea gDNA ka lisele tsa lero la mali, serum le 'mele, ho khothaletsoa TIANamp Micro DNA Kit. Bakeng sa tlhoekiso ea vaerase gDNA ho tsoa disampoleng tsa serum / plasma, ho khothaletsoa TIANamp Virus DNA / RNA Kit. Bakeng sa tlhoekiso ea baktheria gDNA ho tsoa disampoleng tsa serum le plasma, ho khothaletsoa TIANamp Bacteria DNA Kit (lysozyme e lokela ho kenyelletsoa bakeng sa baktheria e ntle). Bakeng sa mehlala ea mathe, Hi-Swab DNA Kit le TIANamp Bacteria DNA Kit lia khothaletsoa.

    P: U ka khetha lisebelisoa joang bakeng sa ho ntšoa ha gDNA ho mehlala ea li-fungus?

    Sesebelisoa sa Semela sa DNAsecure kapa Sisteme ea Semela sa DNAquick se khothalelitsoe molemong oa ho ntša fungal genome. Bakeng sa ho ntša tomoso ea genome, ho khothalletsoa TIANamp Yeast DNA Kit (lyticase e lokela ho itokisetsa).

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