FastFire qPCR PreMix (SYBR Green)

SYBR Green fluorescent ea reagent e ngata ka ho fetisisa.

FastFire qPCR PreMix e sebelisa anti-anti e fetotsoeng Anti Taq DNA polymerase le sistimi e ikhethileng ea PCR Buffer ho netefatsa karabelo e bonolo le ea qPCR ho lisebelisoa tsohle tsa Real-Time PCR. E na le litšobotsi tsa karabelo e potlakileng, ka nako e khuts'oane ea 60R ea karabelo ea PCR, ts'ebetso e phahameng ea kholiso, bophahamo bo phahameng ba kholiso le mefuta e mengata e tšepahalang, e nolofalletsang pokello ea sephetho ka potlako ntle le ho ama phello ea PCR.

Katse. Che	Paka Size
4992217	20 ×l × 125 rxn
4992249	20 ×l × 5000 rxn
4992218	20 ×l × 500 rxn

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Likaroloana

■ E potlakileng ka ho fetisisa: Antibody e fetotsoe Anti Taq DNA polymerase, e sebelisana 'moho le sistimi e ikhethileng ea Buffer, e boloka ho fihlela ho 60% ea nako ea karabelo le ho ba reagent e potlakileng ka ho fetesisa ea SYBR' marakeng hajoale.
■ Bokhoni bo matla ba ho holisa matla: Letšoao le matla la ho phahamisa khanya, liphetho tse nepahetseng le tse tšepahalang.
■ Ho ba le botsitso bo botle: Ho tsitsisoa le ho matlafatsa PCR ka mokhoa o ikhethileng ho kenyelelitsoe Buffer, ho etsa hore sephetho se tsitse, se phetehe, se nepahale hape se tšepahale.
■ E sebetsa ka bophara: Ha e loketse feela lisebelisoa tse potlakileng tsa nako ea PCR, empa hape e loketse lisebelisoa tse tloaelehileng tsa nako ea sebele ea PCR.
■ Tokiso ea ROX: Dae ea ROX e kentsoe ka thoko, e leng bonolo ho e sebelisa ebile e ka netefatsa litholoana tse nepahetseng.

Ga tlhaloso

Mofuta: DNA polymerase ea anti -ody-modified e fetotsoeng ke Antibody, SYBR Green I
Nako ea ts'ebetso: ~ 30 min

Likopo: QPCR e nang le dae bakeng sa phumano ea liphatsa tsa lefutso ho DNA kapa tlhahlobo e lekanyelitsoeng ea polelo ho lisampole tsa cDNA tse tsoang mefuteng e fapaneng

Lihlahisoa tsohle li ka fetoloa bakeng sa ODM / OEM. Bakeng sa lintlha,ka kopo tlanya Customized Service (ODM / OEM)

E fetileng: SuperReal PreMix Plus (Phuputso)

E 'ngoe: FastFire qPCR PreMix (Phuputso)

	Bokhoni bo matla ba ho holisa matla - Letšoao le matla la fluorescence E potlakileng-ntlafatsa ntlafatso ea liteko, ho lelefatsa nako ea ts'ebeletso ea sesebelisoa le ho boloka matla Setso DNA polymerase e ka kengoa ts'ebetsong ka potlako, ea netefatsa hore lihlahisoa tsa FastFire li fetoha mofuta o potlakileng oa SYBR Green reagent 'marakeng hajoale ka sistimi e ikhethileng ea Buffer ho ntlafatsa ts'ebetso ea liteko. SYBR Green reagent e potlakileng hajoale, e khutsufatsa nako ea karabelo ka 1000 sec.
	Bokhoni bo matla ba ho holisa matla - Letšoao le matla la fluorescence Letšoao le khanyang la fluorescence le matla ho feta (matla a ho matlafatsa a matla), a nang le mothinya o tloaelehileng oa ho matlafatsa, ho ba le kutlo e phahameng, 'me o khona ho lemoha liphatsa tsa lefutso tsa template e tlase. Letshwao la phumano ya sehlahiswa se amehang ho Mofani wa thepa T le fokola, mme boleng ba CT bo morao, e leng se ka lebisang liphethong tse sa nepahalang.
	Liteko tse 96 tse tsamaeang hantle tse tšoanang li bonts'a hore reagent e na le botsitso bo matla le ho pheta-pheta hamolemo.