EndoFree Maxi Plasmid Kit Featured Image

Kit ea EndoFree ea Maxi Plasmid

Bakeng sa tlhoekiso ea grade ea plasmid e se nang phetoho ea endotoxin.

EndoFree Maxi Plasmid Kit e ipapisitse le theknoloji ea lera la silica ho ntša endasmine e se nang plasmid. Setsi sena se fana ka ts'ebetso e ikhethileng ea ho tlosa li-endotoxin ho hlahisa DNA ea plasmid ea boleng bo holimo bakeng sa lits'ebetso tse tlase. E etselitsoe ho arola plasmid DNA ea bohloeki bo phahameng ho fetisisa ho tloha ho 100-200 ml ea moetlo oa baktheria mme e ka fana ka 1.5 mg transasmion grade plasmid DNA.

Katse. Che	Paka Size
4992194	10 li-preps

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Likaroloana

■ Kotulo e potlakileng le e phahameng: 200 μg-1.5 mg plasmid DNA e hlahisa ka hora e le 'ngoe e nang le karolo e phahameng ea sebopeho se ikharileng.
■ Bohloeki bo phahameng: DNA e hloekileng ea plasmid e fumanoa ka sistimi e ikhethang ea buffer le Spin Column CP6.
■ Ts'ebetso e ntle ea phetisetso: E loketse liteko tsa phetisetso ea lisele tse ngata.
■ Likopo tse fapaneng: E loketse thibelo ea enzyme e thibelang, phetoho, tatellano, microinjection, ho khutsisa liphatsa tsa lefutso le liteko tsa phetisetso.

Tlhahiso ea Plasmid

Plasmid Yield

Lihlahisoa tsohle li ka fetoloa bakeng sa ODM / OEM. Bakeng sa lintlha,ka kopo tlanya Customized Service (ODM / OEM)

E fetileng: TIANprep ka potlako N96 Plasmid Kit

E 'ngoe: EndoFree Mini Plasmid Kit II

Tlhahiso e phahameng / e tlase ea plasmid
Plasmid pBR322 e nang le kopi e tlase, e nkiloeng moetlong oa baktheria oa 200 ml o sebelisang EndoFree Maxi Plasmid Kit, e ile ea phahamisoa ho 1 ml Buffer TB, e nang le 0.6 μg / μl. PBS e nang le kopi e phahameng, e nkiloeng moetlong oa baktheria oa 100 ml e sebelisang EndoFree Maxi Plasmid Kit, e ile ea phahamisoa ho 1 ml Buffer TB, e nang le 1,2 μg / μl.
MIV: TIANGEN DNA Letšoao IV
pBR322: 2 μl, pBS: 2 μl

pEGFP e fumanoeng ka EndoFree Maxi Plasmid Kit e ile ea fetisetsoa ka thoko ho sele ea endotoxin-insensitive cell line 293T le cell cell-sensitive cell line Huh7 ka TIANGEN TIANfect. Tlhaloso ea GFP e fumanoe lihora tse 24 kamora phetisetso.

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