TIANprep ka potlako N96 Plasmid Kit

Phallo e phahameng, tlhahiso e potlakileng ea palo e nyane ea plasmid.

Thepa e sebelisa mokhoa oa pula oa isopropanol ho fumana plasmid DNA. Thepa e amohela 96-hantle filtara poleiti N le Buffer III, tse khethehileng ntshetswa pele ke TIANGEN, 'me ka ka potlako le ka tsela e atlehileng ntša phahameng-hloekileng plasmid DNA. E loketse ho lokisoa ka potlako ha li-plasmids tse nang le lisebelisoa tse phahameng. 1.3 ml ea setso sa baktheria ea plasmid e nang le kopi e phahameng e ka sebetsoa selibeng se seng le se seng, 'me lisampole tse 96 tsa plasmid DNA li ntšoa kapele. Sehlahisoa se phahameng ka ho fetisisa selibeng ke 10-20 μg (chai e amana le lintlha tse kang palo ea plasmid kopi, boemo ba baktheria, jj.). Plasmid DNA e ntšitsoeng ka har'a kit e ka sebelisoa litekong tsa baeloji ea limolek'hule tse kang tšitiso ea enzyme, tatelano, tlhahlobo ea laeborari, ligation le phetoho.

Katse. Che:	Paka Size
4992868	4 pele
4992869	24 pele

Romela ya imeile ho re

Product Qaqileng

Phallo ea mosebetsi

Mohlala oa Teko

Lihlahisoa tsa Product

Likaroloana

■ E bonolo ebile e bonolo: Mehlala ea 96 e ka nkuoa ka lihora tse 2-3.
■ Ho hlomelloa ka reagent e ikhethang ea TIANRed, e ka supa ka ho hlaka boemo ba lysis ea sampole ts'ebetsong ea ho ntša metsi ho etsa bonnete ba hore DNA ea plasmid e hloekile haholo.
■ Bohloeki bo phahameng: Plasmid e nang le bohloeki bo phahameng e ka sebelisoa ka kotloloho litekong tse tlase.

Lihlahisoa tsohle li ka fetoloa bakeng sa ODM / OEM. Bakeng sa lintlha,ka kopo tlanya Customized Service (ODM / OEM)

E fetileng: Kit ea EndoFree Midi Plasmid

E 'ngoe: Kit ea EndoFree ea Maxi Plasmid

TIANprep Rapid N96 Plasmid Kit01

	Plasmid DNA e ntšitsoe ho 1 ml ea moro oa Escherichia coli (TOP10) oa bosiu bo le bong (LB medium) o sebelisa TIANprep Rapid N96 Plasmid Kit, mme karolelano ea 8-10 μg ea bohloeki bo phahameng pBS plasmid e fumanoe. 2 µl plasmid e ile ea kenngoa ka selibeng ka seng sa 1% ea gel ea agarose, 'me ea fumanoa ke electrophoresis.
	Plasmid DNA e ntšitsoe ho 1 ml ea moro oa Escherichia coli (TOP10) oa bosiu bo le bong (LB medium) o sebelisa TIANprep Rapid N96 Plasmid Kit, mme karolelano ea 8-10 μg ea bohloeki bo phahameng pBS plasmid e fumanoe. Vector: pBS plasmid; Mathata: TOP10. Plasmid e ne e hlophisitsoe ke ABI. PRISM 3730XL DNA sequencer