TIANGEN e fane ka liteko tse fetang limilione tse 100 bakeng sa ASFV
Tharollo ena e ka sebelisoa bakeng sa ho lekola mafu nakong ea tlhahiso ea likolobe le ho sibolla vaerase nakong ea polokelo ea nama ea kolobe le ts'ebetso.
Re ka fana ka tharollo e fapaneng ea phumano bakeng sa lisele, mali, setuloana hape
e le disampole tikoloho.
Sample Preservation
Mohlala oa Peleho
Ho ntša metsi ka nyutlelie
Tharollo ea RT-qPCR
Molomo oa Swab Mohlala oa ho Boloka Buffer
RNAstore e ikemiselitse
Sireletsa RNA ho se nang serame
Kopo: Ho boloka boko, pelo, liphio, spleen, sebete, matšoafo le thymus, jj.
E boloka RNA: 1 letsatsi ho 37 ° C, matsatsi a 7 ho 15-25 ° C, kapa libeke tse 4 ho 2-8 ° C. Polokelo ea nako e telele ho -20 ° C kapa -80 ° C.
Dinama tse nyenyane tse sampole sila
Tharollo ea homogenization
Lisele tsa Sissue Sissue
E loketse ho ntšoa ha DNA / RNA / protheine ea lisele tsa limela / tsa liphoofolo, mobu, mantle, li-fungus, jj.
Sebetsa mocheso: ka tlaase ho -10 ℃
Phallo: Mehlala ea 1-24
Phello ea RNA ea liphoofolo e ntšitsoeng kamora ho sila ke H24R
Sampole ea chelete: 20 mg (pelo: 10mg) Sample: Crucian carp Marker: DNA MarkerIII, TIANGEN, MD103-02, RNA Extraction Kit: RNA Easy Fast Tissue / Cell Kit (TIANGEN, 4992732) RNA e ntšitsoeng kamora ho sila ke TGrinder H24R ke ea mahloriso a phahameng le bohloeki.
Sehlahisoa se iketselitseng sa Nucleic Acid Extractor
● Litsela tse 32 le tse 96 ha li ikhethele.
● Ho ntša ka potlako kokoana-hloko ea nucleic acid nakong ea metsotso e 30.
● Lisebelisoa tsa reagent tsa boleng bo holimo tse fumanehang bakeng sa ts'ebetso e nepahetseng.
Tharollo e Bulehileng e Phahameng
● Tšebelisano e phahameng, e lumellanang hantle le senoko se tloaelehileng sa nucleic acid 'marakeng.
● Ho paka ka mokhoa o ikhethileng le litšebeletso tsa OEM li entsoe ho latela litlhoko tse ikhethileng.
● E lumellana: KingFisher, Hamilton, Beckman Coulter, Chemagen jj.
Tharollo ea Buka ea Tlhahiso
TIANamp Virus DNA / RNA Kit
● Ke lisebelisoa tse bonolo feela tse hlokahalang ho phethela teko ea ho ntša lintho
● Lisebelisoa tse sebelisoang ha li rekisoa li arohane ka thoko ho qoba tšilafalo
● Nako e khuts'oane ea ho ntša le ts'ebetso e bonolo, e nang le ts'ebetso e phahameng.
● Ho paka ka mokhoa o ikhethileng le litšebeletso tsa OEM li entsoe ho latela litlhoko tse ikhethileng.
● Ho na le li-enzyme tse matla tsa RT, qPCR tse fanoang ka sistimi e ntlafalitsoeng ea karabelo.
● Boholo ba li-template bo ka khetholloa ka nepo.
● Ho paka ka mokhoa o ikhethileng le litšebeletso tsa OEM li entsoe ho latela litlhoko tse ikhethileng.
Probe-based real time PCR reagent / raw enzyme / ODM / OEM
Km gana Pipetting System (itingoe lijo 'me phahameng throughput km gana itšoara joang ho seta)
Phallo: Litsela tse le 'ngoe kapa tse 8
Kopo: PCR kapa qPCR setup reaction
