TIANprep N96 Plasmid Kit

Tlhokomelo ea nako ea 'nete ea boemo ba lysis le ho qhekelloa ho phahameng ha li-plasmids tse hloekileng

Lera la N96 Plate CP3 ka har'a sesebelisoa sena le amohela sesebelisoa sa silica matrix ka mokhoa o ikhethileng o ntlafalitsoeng ke TIANGEN, e ka hlahisang DNA ea plasmid hantle ka nepo, ea tlosa liprotheine le litšila tse ling haholo, ka hona ea netefatsa ho hloeka ha plasmid e ntšitsoeng. Ho fihlela 1.3 ml ea moetlo oa baktheria ea plasmid e ka sebetsoang selibeng se seng le se seng, ho tloha ho eona ho fihlela 5-10 μg ea plasmid DNA e ka ntšoa ka potlako (chai e amana le lintlha tse kang palo ea kopi ea plasmid, boemo ba baktheria, jj. ). Plasmid DNA e ntšitsoeng ka har'a kit e ka sebelisoa litekong tsa baeloji ea limolek'hule tse kang tšitiso ea enzyme, tatelano, tlhahlobo ea laeborari, ligation le phetoho.

Katse. Che	Paka Size
4992892	Li-preps tse 4
4992893	24 li-preps

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Likaroloana

■ Lebelo le potlakileng la ho ntša.
■ Ho hlomelloa ka reagent e ikhethang ea TIANRed, e ka supa ka ho hlaka boemo ba lysis ea sampole ts'ebetsong ea ho ntša metsi ho netefatsa ts'ebetso e phahameng le bohloeki bo phahameng ba DNA ea plasmid.
■ Ho hloeka ho hoholo: Plasmid DNA e hloekisitsoeng e ka sebelisoa ka kotloloho litekong tsa noka.
■ Seteishene sena se ka sebelisoa litsing tsa tšebetso tse phahameng tsa motlakase tse hlahisang li-acid.

Letšoao le khubelu la TIANRed

TIANRed Color indicator:

Lihlahisoa tsohle li ka fetoloa bakeng sa ODM / OEM. Bakeng sa lintlha,ka kopo tlanya Customized Service (ODM / OEM)

E fetileng: TIANamp FFPE DNA Kit

E 'ngoe: TIANamp Mali a DNA Midi Kit

	Plasmid DNA e ntšitsoe ho 1 ml ea moro oa Escherichia coli (TOP10) oa bosiu bo le bong (LB medium) o sebelisa TIANprep Highpure N96 Plasmid Kit, mme karolelano ea 5-6 μg ea bohloeki bo phahameng pBS plasmid e fumanoe. Plasmid e ne e kentsoe ka selibeng se seng le se seng sa gel ea 1% ea agarose, mme e fumanoe ke electrophoresis
	Plasmid DNA e ntšitsoe ho 1 ml ea moro oa Escherichia coli (TOP10) oa bosiu bo le bong (LB medium) o sebelisa TIANprep Highpure N96 Plasmid Kit, mme karolelano ea 5-6 μg ea bohloeki bo phahameng pBS plasmid e fumanoe. Vector: pBS; Mathata a Plasmid: TOP10. Plasmid e ne e hlophisitsoe ke ABI. PRISM 3730XL DNA sequencer.