TIANamp Mobu oa DNA Kit

Ho ntša ka potlako DNA ea genomic mefuteng e fapaneng ea mobu.

TIANamp Soil DNA Kit e sebelisa sistimi e ikhethileng ea litšepe, eo asiti ea humic e sampoleng ea mobu e ka tlosoang ka botlalo. Lihlaha tsa Zirconium li boetse li sebelisoa ka kiteng ena ho sebetsana le lysis ea likarolo tsa mehlala ea mobu e le ho netefatsa ho kopanya ha gDNA.

Katse. Che Paka Size
4992288 50 li-preps

Product Qaqileng

Mohlala oa Teko

LBH

Lihlahisoa tsa Product

Likaroloana

■ Kopo e pharalletseng: Khetholla DNA e hloekileng mehlala e fapaneng ea mobu, joalo ka mobu oa lipalesa, mobu oa pitsa, mobu oa masimo, mobu oa moru, sludge, mobu o mofubelu, mobu o motšo, lerōle le mefuta e meng e mengata ea mobu.
■ Protocol e potlakileng: Tsamaiso eohle ea liteko e ka phetheloa ka nako e khuts'oane.
■ Bohloeki bo phahameng: Ts'ebeliso ea kholomo ea spin e ka netefatsa ho hloeka ho hoholo ha DNA, e ka sebelisoang ka kotloloho litekong tse tlase.

Likopo

■ DNA e ntšitsoeng ka har'a sesebelisoa sena e na le litšila tse fokolang ebile e na le ts'epahalo e phahameng, e ka sebelisoang ka kotloloho litekong tsa biology tsa molek'hule tse tlase tse kang PCR le tšitiso ea tšilo ea lijo.

Lihlahisoa tsohle li ka fetoloa bakeng sa ODM / OEM. Bakeng sa lintlha,ka kopo tlanya Customized Service (ODM / OEM)


  • E fetileng:
  • E 'ngoe:

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    Experimental Example Genomic DNA e hloekisitsoe mefuteng e fapaneng ea mobu ke TIANamp Soil DNA Kit. Sampole ea chelete: 250 mg; 5 μl ea li-elux tse 50 μl li ile tsa laeloa ka leleng le leng ho 1% ea gel ea agarose.
    1. Mobu o motšo oa Hei Long Jiang; 2. Laterite ho tloha Guangdong;
    3. Loess oa Beijing; 4. Laterite ho tloha Zhejiang;
    5. Hamorao ho tsoa Yunnan; 6. Lerōle la laboratori;
    7. Mobu oa meru; 8. Sludge;
    9. Mobu oa masimong; 10. Ho bopa mobu;
    11. Mobu oa bethe ea lipalesa
    Experimental Example Genomic DNA e hloekisitsoeng mecheng e fapaneng ea mobu e sebelisang TIANamp mochini oa DNA oa mobu o ile oa lekoa ke kholiso ea PCR. 6 μl ea lihlahisoa tsa PCR tse 20 μl li ile tsa beoa tseleng e 'ngoe le e' ngoe.
    1. Mobu o motšo oa Hei Long Jiang; 2. Laterite ho tloha Guangdong;
    3. Loess oa Beijing; 4. Laterite ho tloha Guangxi;
    5. Lerōle la laboratori; 6. Mobu oa meru;
    7. Ho bopa mobu; 8. Mobu oa bethe ea lipalesa;
    9. Laterite ho tloha Zhejiang; 10. Sludge;
    11. Mobu oa masimong;
    M: TIANGEN Lesupa III
    NTC: taolo e mpe ntle le litempele
    P: DNA e nyane kapa ha e eo ho lona.

    A-1 Lisele tsa vaerase kapa vaerase e tlase sampoleng e qalang -E ruisa bongata ba lisele kapa livaerase.

    A-2 Lysis e sa lekanang ea lisampole -Sampole ha e so tsoakane hantle le buffer ea lysis. Ho khothalletsoa ho kopanya ka botlalo ka pulse-vortexing makhetlo a 1-2. —Leseli e sa lekanang ea lisele e bakoang ke ho fokotseha ha tšebetso ea proteinase K. Ho khothalletsoa ho seha lisele ka likotoana tse nyane ebe o eketsa nako ea ho hlapa ho tlosa masalla a lysate.

    A-3 Phatlalatso e sa lekaneng ea DNA. -Ha ho ethanol kapa liperesente tse tlase ho e-na le 100% ea ethanol e ileng ea eketsoa pele lysate e fetisetsoa ho kholomo ea spin.

    A-4 Boleng ba pH ba elution buffer bo tlase haholo. - Fetola pH hore e be lipakeng tsa 8.0-8.3.

    P: DNA ha e sebetse hantle litekong tsa enzymatic reaction reaction.

    E setseng ea ethanol ka eluent.

    -Ho na le masalla a hlatsoang sesupa-tsela PW lehloeng. Ethanol e ka tlosoa ka ho kenya centrifuging spin ea kholomo bakeng sa 3-5 min, ebe e beha mocheso oa kamore kapa 50 ℃ incubator bakeng sa 1-2 min.

    P: Ho senyeha ha DNA

    A-1 Sampole ha e ncha. —Khetholla sampole e ntle ea DNA e le taolo ea ho bona hore na DNA e samporeng e senyehile.

    A-2 Pele ho kalafo e fosahetseng. —E bakoa ke ho sila metsi a naetrojene e feteletseng, ho fumana mongobo hape kapa sampole e ngata haholo.

    Q: U ka etsa pele joang kalafo ea tlhahiso ea gDNA?

    Li-prereatment li lokela ho fapana bakeng sa mehlala e fapaneng. Bakeng sa mehlala ea semela, etsa bonnete ba hore u sila ka botlalo ka naetrojene ea metsi. Bakeng sa disampole tsa liphoofolo, homogenate kapa sila ka botlalo ka naetrojene ea metsi. Bakeng sa mehlala e nang le mabota a sele eo ho leng thata ho e roba, joalo ka baktheria ea G + le tomoso, ho kgothaletswa hore ho sebelisoe lysozyme, lyticase kapa mekhoa ea mochini ho roba mabota a sele.

    P: Phapang ke efe lipakeng tsa lisebelisoa tse tharo tsa tlhahiso ea limela tsa gDNA 4992201/4992202, 4992724/4992725, 4992709/4992710?

    4992201/4992202 Plant Genomic DNA Kit e sebelisa mokhoa o sebelisang kholomo o hlokang chloroform bakeng sa ho ntšoa. E loketse haholo-holo mefuta e fapaneng ea limela, hammoho le phofo e ommeng ea semela. Hi-DNAsecure Plant Kit le eona e thehiloe holima kholomo, empa ha ho hlokahale hore e ntšoe phenol / chloroform, ee boloke e se na chefo. E loketse limela tse nang le polysaccharides e phahameng le polyphenol. 4992709/4992710 DNAquick Plant System e sebelisa mokhoa o sebelisang metsi. Phenol / chloroform ha e hlokehe hape. Tsamaiso ea tlhoekiso e bonolo ebile e potlakile e se na moeli bakeng sa litekanyo tsa sampole e qalang, kahoo basebelisi ba ka fetola chelete ka mokhoa o feto-fetohang ho latela litlhoko tsa liteko. Boholo bo boholo ba likhechana tsa gDNA bo ka fumaneha ka chai e ngata.

    Ke tlhahiso efe e hakantsoeng ea gDNA ho tsoa ho 1 ml sampole ea mali ke TIANamp Blood DNA Kit?

    DNA ea genomic e ntšitsoe mehatong e fapaneng ea lisele tsa motho ka botlalo ke TIANamp Blood DNA Kit. Liphetho li latela. Liphetho li thathamisitsoe e le litšupiso feela, liphetho tsa 'nete tsa ho ntša metsi li latela maemo a mehlala.

    faq

    P: Na 4992207/4992208 le 4992722/4992723 li ka sebelisoa ho ntša mali a DNA?

    Ho ntšoa ha DNA ea mali a mali ho ka etsoa ho sebelisoa li-reagents tse fanoeng ka har'a lisebelisoa tsena tse peli ka ho fetola protocol ho latela taelo e ikhethang ea ho ntšoa ha mali a DNA. Kopi e bonolo ea protocol ea ho ntša mali ea clot ea mali e ka fanoa ha ho kopuoa.

    P: Ha u sebelisa TIANamp Genomic DNA Kit, u ka roba lisele tse ncha hore li fanyehe joang?

    Emisa sampole e ncha ka 1 ml PBS, letsoai le tloaelehileng kapa buffer ea TE. Ka ho feletseng homogenize sampole ke homogenizer le bokella precipitate ho ea tlase ea tube, ke centrifuging. Lahla matla a phahametseng tlhaho, 'me u nchafatse sepakapaka ka 200 μl buffer GA. Tlhoekiso e latelang ea DNA e ka etsoa ho latela taelo.

    P: U ka khetha sehlahisoa sefe bakeng sa ho ntšoa ha DNA ho tsoa liseleng tsa plasma, serum le mokelikeli oa 'mele?

    Bakeng sa tlhoekiso ea gDNA ka lisele tsa lero la mali, serum le 'mele, ho khothaletsoa TIANamp Micro DNA Kit. Bakeng sa tlhoekiso ea vaerase gDNA ho tsoa disampoleng tsa serum / plasma, ho khothaletsoa TIANamp Virus DNA / RNA Kit. Bakeng sa tlhoekiso ea baktheria gDNA ho tsoa disampoleng tsa serum le plasma, ho khothaletsoa TIANamp Bacteria DNA Kit (lysozyme e lokela ho kenyelletsoa bakeng sa baktheria e ntle). Bakeng sa mehlala ea mathe, Hi-Swab DNA Kit le TIANamp Bacteria DNA Kit lia khothaletsoa.

    P: U ka khetha lisebelisoa joang bakeng sa ho ntšoa ha gDNA ho mehlala ea li-fungus?

    Sesebelisoa sa Semela sa DNAsecure kapa Sisteme ea Semela sa DNAquick se khothalelitsoe molemong oa ho ntša fungal genome. Bakeng sa ho ntša tomoso ea genome, ho khothalletsoa TIANamp Yeast DNA Kit (lyticase e lokela ho itokisetsa).

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