Ka December 2019, letoto la linyeoe tsa pneumonia tse sa tsejoeng li qalile ho tloha Wuhan, Profinseng ea Hubei, le haufinyane e ile ea namela liprofinseng le metseng e mengata ea Chaena, le linaheng tse ling tse ngata ka Pherekhong 2020. Ho tloha ka 22:00 bosiu ka Pherekhong 27, linyeoe tse 28 tse netefalitsoeng le linyeoe tse 5794 tse belaelloang tsa 2019-nCov li tlalehiloe China. Thetšoaetso mohloli oa vaerase e ne e tšoauoa joalo ka Rhinolophus, mme sekhahla sa lefu la hajoale ke 2.9%.
Ka la 12 Pherekhong 2020, Mokhatlo oa Lefatše oa Bophelo (WHO) o phatlalalitse hore seoa sa pneumonia se bakiloe ke mofuta o mocha oa coronavirus (2019-nCov). Coronavirus ke mofuta oa vaerase e jalang har'a liphoofolo. Mofuta oa vaerase ea acid ea nucleic ke RNA e le 'ngoe. Ka nako e ts'oanang, WHO e boetse e lokolotse tlhaiso-leseling ea li-acid tsa nucleic ea nCov e arolelanoang ke litsebi tsa China, e faneng ka metheo ea ho fumana limolek'hule bakeng sa ho sibolla vaerase mme e entse hore ho fumanoe kapele le ho khetholla likokoana-hloko.
WHO e fana ka maikutlo a hore batho ba nang le mafu a hlobaetsang a ho hema a kang ho khohlela, feberu, tšoaetso ea tšoaetso ea phefumoloho mme ba bile Wuhan nakong ea matsatsi a 14 kapa ba pepeselitsoe ho kopana le bakuli ba bang ba lokela ho hlahlojoa ka botlalo. Ka la 17 Pherekhong 2020, WHO e ile ea lokolla "liteko tsa laboratori bakeng sa korona ea coronavirus ea 2019 (2019-nCoV) maemong a belaelloang a batho, tataiso ea nakoana, la 17 Pherekhong 2020". Tataiso e supa hore mehlala e lokelang ho bokelloa ho tsoa ho bakuli ba nang le matšoao a tšoaetso e kenyelletsa mehlala ea phefumoloho (nasopharynx le oropharyngeal swabs, sekhohlela, bronchoalveolar lavage, jj.) Le lisampole tsa serum, ka tsela e latelang:
* Bakeng sa ho tsamaisa disampole bakeng sa phumano ya vaerase, sebedisa VTM (maqhubu a dipalangwang tsa vaerase) e nang le di tlatsetso tsa ho lwantsha mafung le dithibela-mafu. Bakeng sa moetlo oa baktheria kapa fungal, lipalangoang li omme kapa ka metsi a nyopa haholo. Qoba ho pheta leqhoa le ho qhibiliha ha lipapiso.
Ntle le lisebelisoa tse ikhethileng tsa pokello tse bontšitsoeng tafoleng hape li netefatsa lisebelisoa tse ling le lisebelisoa li teng: mohlala, lijana tsa lipalangwang le mekotla ea pokello ea lisampole le liphutheloana, lintho tse pholileng le lipakete tse batang kapa leqhoa le ommeng, lisebelisoa tse se nang mali tsa ho hula mali (mohlala, linalete, lisirinji le lipeipi), dileibole le matšoao a sa feleng, PPE, lisebelisoa tsa ho tlosa litšila bokaholimo.
Bakeng sa ho sibolla vaerase, mehlala ea serum e ka sebelisoa ho lemoha vaerase ka mokhoa oa ho itšireletsa mafung, ha RTqPCR e khothalletsoa ho fumana nCov nucleic acid bakeng sa ho lemoha ka potlako le ka nepo. Joaloka vaerase
ho latellana hoa tsebahala, likahare tsa bongata ba kokoana-hloko ea nucleic li ka fumanoa kapele feela
ho khetha li-reagents tse loketseng le li-primer tse tšoanang
(Tlhatlhobo ea Wuhan coronavirus 2019 ke RTPCR, Protocol le tlhahlobo ea pele ho nako ka la 13 Pherekhong 2020) Joaloka moetapele lebaleng la ho qhekelloa le ho sibolloa ha acid e Chaena, TIANGEN BIOTECH (BEIJING) CO., LTD. e fane ka kokoana-hloko ea molomo oa maoto le molomo oa ntaramane A (H1N1) reagents bakeng sa batho ba fetang limilione tse 10. Ho 2019, li-reagents tsa TIANGEN tsa kokoana-hloko ea li-virus le li-reagents li sebelisitsoe ho lisampole tsa Afrika tsa likolobe tsa likolobe tse limilione tse 30, ho etsa tlatsetso e hlahelletseng ho fumanoeng le thibelong ea feberu ea likolobe ea Afrika e Chaena. TIANGEN ha e fane feela ka lisebelisoa tse potlakileng le tse nepahetseng tsa ho ntša vaerase le ho e fumana, empa hape e fana ka lisebelisoa tse bobebe le tse sebetsang tsa ho sila lisele le lisebelisoa tse iketsang tsa nucleic acid, tse bopang tharollo e felletseng ea ho ntša vaerase le ho e fumana.
Tharollo ea Tharollo ea Nucleic Acid
TIANGEN Automated Nucleic Acid Extractors ke li-platform tsa othomathike tsa ho ntša nucleic acid li sebelisa mokhoa oa matla a khoheli. Ts'ebeliso ea sethala sena ha se fokolise feela boima ba mosebetsi ba mafapha a ho lekola le ho arola batho ba bang, empa hape se fokotsa liphoso tsa ts'ebetso ea ho ntšoa ka letsoho, hape e netefatsa botsitso le botsitso ba boleng ba acid e hlahisitsoeng ea nucleic.
TIANGEN Automated Nucleic Acid Extractor e na le lisebelisoa tse fapaneng (ho kenyeletsoa liteishene tsa 16, 24, 32, 48, 96), 'me li-reagents tse tšoanang li ka sebelisoa bakeng sa ho ntša nucleic acid mefuteng e fapaneng ea mefuta. TIANGEN e boetse e fana ka lits'ebetso tsa ntlafatso ea reagent le lits'ebeletso tsa kopanyo ea lisebelisoa ho latela litlhoko tse fapaneng tsa liteko.
TGrinder H24 dinama tse nyenyane tse Homogenizer
● E sebelisoa haholo ho sila le ho homogenizing mefuta e fapaneng ea lisele le mantle
● Ka matla a ho sila a homogenization makhetlo a 2-5 ho feta a lisebelisoa tsa setso
● Ho sila le ho etsa homogenifomo e le 'ngoe, ho qoba tšilafalo ea sefapano
● Sesebelisoa sa tšireletso se ikemetseng ho sireletsa polokeho ea basebetsi ba laboratori
TGuide S32 E itirile ka Nucleic Acid Extractor
● Mohlala oa phallo: Mehlala ea 1-32
● Ho sebetsana le bophahamo ba modumo: 20-1000 μl
● Mofuta oa mohlala: Mali, lisele, lisele, mantle, vaerase le lisampole tse ling
● Nako ea ho sebetsana: Ho fihlela ho metsotso e 8 ho fumana acid e nang le vaerase
● Mokhoa oa ho laola: Mokhoa o habeli oa taolo ea Windows Pad le konopo ea skrini
● Nts'etsopele ea sethala: Sethala se bulehileng, se lokolohileng ho lekana li-reagents
● TGuide S32 Magnetic Viral DNA / RNA Kit (DP604)
● Ts'ebeliso e pharalletseng: DNA / RNA ea vaerase ea boleng bo holimo e ka hloekisoa ho tsoa serum, lero la mali, sampole ea swab, tharollo ea kalafo ea lisele le tharollo e fapaneng ea ho boloka vaerase.
● E bonolo ebile e sebetsa hantle: Sehlahisoa sena se tsamaisana hantle le TGuide S32 Automated Nucleic Acid Extractor, e ka ntšang vaerase ea DNA / RNA ka lihlahisoa tse ngata, bohloeki bo phahameng, boleng bo tsitsitseng le bo tšepahalang.
● Likopo tse tlase tlase: Nice acid e hloekisitsoeng e loketse liteko tse tlase tsa virus ea PCR le PCR ea nako ea nnete.
Tharollo ea Manual Nucleic Acid Extraction
Joaloka moetapele lebaleng la ho qhekelloa le ho sibolloa ha acid e acid, TIANGEN e na le letoto la lihlahisoa tsa ho hlahisa acid e Chaena, tse loketseng ho sibolloa ha coronavirus mefuteng eohle ea mehlala: mali, serum / lero la mali, lisele, swab, vaerase jj.
TIANamp Virus DNA / RNA Kit (DP315)
● Bokhabane bo phahameng: DNA le RNA ea vaerase ea boleng bo holimo e ka fumanoa ka tlhoekiso e potlakileng ka ho phetoa haholo.
● Bohloeki bo phahameng: Ho tlosa lintho tse silafatsang le li-inhibitors ka botlalo.
● Tšireletseho e phahameng: Ha ho hlokahale hore motho a ntše reagent e tsoang ka nko kapa pula ea ethanol.
Tharollo ea tlhahlobo ea vaerase ea RNA
1. Sistimi e Ikemetseng ea Pipetting
● Ho nepahala ho phahameng: Sebaka se pholileng se ka boloka mocheso oa sampole ea reagent e ka tlase ho 7 ℃ bakeng sa metsotso e fetang 60. APM e 'ngoe le e' ngoe e lekantsoe ho latela maemo a machabeng, a nang le ts'ebetso e nepahetseng ho feta pompo ea letsoho.
● Ts'ebetso e bonolo: Saese e nyane. Boima bo bobebe. Ha ho sesebelisoa se hlokahalang bakeng sa block replacemen. Mokhoa oa ho itukisetsa oa PCR / qPCR o hahiloeng. Ts'ebetso e nolofalitsoeng ea ts'ebetso ea tharollo ea PCR.
● Ts'ebeliso e pharalletseng: E ka sebelisoa bakeng sa pompo ea poleiti ea 96/384, PCR, qPCR, phumano ea liphatsa tsa lefutso le liteko tse ling tsa ts'ebetso e phahameng.
FastKing Mohato o le Mong RT-qPCR Kit (Probe) (FP314)
FastKing One Step RT-qPCR Kit (Probe) (FP314) e ntlafalitsoeng ke TIANGEN ke sesebedisoa sa onestep reverse transcription fluorescence kit se ipapisitseng le mokhoa oa lipatlisiso, o etselitsoeng ka ho khetheha bakeng sa ho sibolla liphatsa tsa lefutso mehlala e fapaneng. KingRTase ka har'a kit ke molek'hule e ncha e fetotsoeng ea reverse transcriptase, e nang le kamano e matla ea RNA le botsitso ba mocheso, e nang le ts'ebetso e ntlafalitsoeng ea phetisetso ea lingoloa le bokhoni ba katoloso ea li-template tsa RNA tse rarahaneng. Qalo e ncha e chesang ea Taq DNA polymerase e boetse e sebelisoa ho fa ts'ebetso ea PCR tšebetso e phahameng haholo le matla. Ntle le moo, kit e nolofatsa likarolo ho isa tekanyong e kholo ka ho kopanya enzyme ea Taq le MLV hore e be motsoako oa enzyme, le pele ho kopanya ion buffer, dNTPs, PCR stabilizer le enhancer ho MasterMix, molemong oa hore mehato ea ho kopanya likarolo tse ngata e ka ba
nolofalitsoe.
● Ts'ebetso e phahameng: Ts'ebetso e ntle ea reverse transcriptase le DNA polymerase e netefatsa tšebetso e phahameng ea tšebetso
● Phetoho e ntle: Polymerase e ka bala template ea RNA e nang le litaba tse phahameng tsa GC le sebopeho se rarahaneng sa bobeli
● Likopo tse pharalletseng: Ho sebetsa haholo ho li-template tsa RNA tse nang le litšila tsa mefuta e fapaneng
● Kutloisiso e phahameng: Litempele tse tlase ho 1 ng li ka tsejoa ka nepo, haholo-holo bakeng sa litempele tse ngata tse tlase
Mohlala oa Phumano ea vaerase ea RNA
Nucleic acid ea H5 feberu ea linonyana e ntšitsoe ke TGuide S32 Magnetic Viral DNA / RNA Kit (DP604). FastKing One Step RT-qPCR Kit (Probe) (FP314) e ne e sebelisetsoa ho fumana RT-qPCR e sebelisa li-primer le li-probes tsa H5 avian influenza.
ABi7500Fast e ne e sebelisetsoa ho fumana RT-qPCR. Liphetho tsa H5 avian influenza virus antigen (10-2, 10-3, 10-4, 10-5, 10-6 le 10-7 dilution) ho tloha ho 200 μl li bonts'a chai e ngata ea kokoana-hloko ea acid, e ka fihlelang se latelang litlhoko tsa phetolelo e fetotsoeng, PCR, RT-PCR, PCR ea nako ea 'nete, jj. Mefuta e fapaneng ea disampole tsa bongata ba kokwanahloko ea acid e ka fumanoa ka nepo.
Nako ea poso: Apr-11-2021
