Theknoloji ea ho hlahloba limolek'hule, haholo-holo tlhahlobo ea likokoana-hloko ea metagenomic (mNGS), e na le menyetla e metle ea ts'ebeliso ea ts'oaetso ea likokoana-hloko, boitsebiso bo bocha bo sa tsejoeng ba likokoana-hloko, ts'oaetso ea tšoaetso ea meriana, tlhahlobo ea ts'oaetso ea lithethefatsi, tlhahlobo ea tšebetso ea batho le tlhahlobo ea ts'oaetso e khahlanong le ts'oaetso. E fana ka mokhoa oa bohlokoa bakeng sa thibelo le taolo ea mafu a tšoaetsanoang, hape e khothaletsa ntlafatso ea bokhoni ba bongaka ba ho hlahloba.
MNGS e ka sekaseka ka mokhoa o hlakileng likokoana-hloko le ho amohela liphatsa tsa lefutso (DNA le RNA) mehlala ea bakuli, 'me butle-butle e tloha laboratoring e ea ts'ebetsong ea bongaka, e le karolo ea motheo oa tlhahlobo ea lingaka, mme e fana ka ts'ehetso bakeng sa tlhahlobo ea sepheo.
TIANGEN e fana ka tharollo ea qalo ea likokoana-hloko tsa mantlha le kaho ea laeborari ea mNGS.
Ho araba seoa sa COVID-19
Ho tloha ha COVID-19 e qhoma, TIANGEN e fane ka liteko tse limilione tse 5 e le lisebelisoa tse tala bakeng sa ho ntša vaerase ea nucleic acid le li-reagents tse ngata tsa ho bona li-fluorescent bakeng sa baetsi ba li-reagent tse fetang 200 le likarolo tsa bonyollo Asia, Leboea le Amerika Boroa le linaheng tsa Europe. Mme e thusa batho ba limilione linaheng tse fetang 30 ho pota lefatše.
Lihlahisoa tsa TIANGEN tsa ho ntša vaerase, e le lisebelisoa tse tala, li ile tsa amoheloa tlalehong ea tlhahlobo mabapi le ts'ebeliso ea tšohanyetso ea COVID-19 e lokollotsoeng ke Mokhatlo oa Lefatše oa Bophelo ka Phuptjane 2020, e ne e thathamisitsoe lenaneng le khothalletsoang la li-reagents tse ncha tsa lefatše tsa COVID-19 tse lokollotsoeng ke Global Fund ka Pherekhong 2021.
Ho tloha lihlahisoa tse tala ho qeta lihlahisoa
maqhama tsohle li tieo laoloa tlas'a tsamaiso ISO13485 boleng ya taolo
Tikoloho ea Tlhahiso
Lisebelisoa tse so fetoloe
Lihlahisoa tsa semi
QC-NGS-E Thehiloe
Sample Preservation
Mohlala oa Peleho
Ho ntša metsi ka nyutlelie
NGS
Sesebelisoa sa molomo sa swab Preservation Buffer
RNAstore e ikemiselitse
Sireletsa RNA ho se nang serame
Kopo: boko, pelo, liphio, spleen, sebete, matšoafo le thymus, jj.
E boloka RNA: 1 letsatsi ho 37 ° C, matsatsi a 7 ho 15-25 ° C, kapa libeke tse 4 ho 2-8 ° C. -20 ° C kapa -80 ° C
Dinama tse nyenyane tse sampole sila
Tharollo ea Homogenization
E loketse ho ntšoa ha DNA / RNA / protheine ea lisele tsa limela / tsa liphoofolo, mobu, mantle, li-fungus, jj.
Sebetsa mocheso: ka tlaase ho -10 ℃
Phallo: Mehlala ea 1-24
Phello ea RNA ea liphoofolo e ntšitsoeng kamora ho sila ke H24R
Sampole ea chelete: 20 mg (pelo: 10mg) Sample: Crucian carp Marker: DNA MarkerIII, TIANGEN, MD103-02, RNA Extraction Kit: RNA Easy Fast Tissue / Cell Kit (TIANGEN, 4992732) RNA e ntšitsoeng kamora ho sila ke TGrinder H24R ke ea mahloriso a phahameng le bohloeki.
Sehlahisoa se iketselitseng sa Nucleic Acid Extractor
● Litsela tse 32 le tse 96 ha li ikhethele.
● Ho ntša ka potlako kokoana-hloko ea nucleic acid nakong ea metsotso e 30.
● Lisebelisoa tsa reagent tsa boleng bo holimo tse fumanehang bakeng sa ts'ebetso e nepahetseng.
Tharollo e Bulehileng e Phahameng
Matla a khoheli Hi-DNA / RNA Kit (4992408 -T4A)
● Tšebelisano e phahameng, e lumellanang hantle le senoko se tloaelehileng sa nucleic acid 'marakeng.
● Ho paka ka mokhoa o ikhethileng le litšebeletso tsa OEM li entsoe ho latela litlhoko tse ikhethileng.
● E lumellana: KingFisher, Hamilton, Beckman Coulter, Chemagen jj.
Nako ea 'nete ea PCR amplifi cation curve ea TIANGEN 4992408
Nako ea 'nete ea PCR amplifi cation curve ea Supplier T
AIV-H5 e ile ea hlakoloa hore e be 10-6 -10-8li-gradients tse nang le metsi a Milli-Q, ebe li ntšoa ke King Fisher Flex. 200 μl ea sampole ka 'ngoe e sebelisitsoe. TIANGEN Kit e bontšitse kutloisiso e ntle le botsitso. Sesebelisoa sa PCR sa 'nete: ABI 7500 Real-time PCR detage reagent: FP314
Tharollo ea Buka ea Tlhahiso
● Ke lisebelisoa tse bonolo feela tse hlokahalang ho phethela teko ea ho ntša lintho
● Lisebelisoa tse sebelisoang ha li rekisoa li arohane ka thoko ho qoba tšilafalo
● Nako e khuts'oane ea ho ntša le ts'ebetso e bonolo, e nang le ts'ebetso e phahameng.
● Ho paka ka mokhoa o ikhethileng le litšebeletso tsa OEM li entsoe ho latela litlhoko tse ikhethileng.
TIANamp Virus DNA / RNA Kit (4992285)
TIANamp Virus RNA Kit (4992286)
TIANamp Micro DNA Kit (4992287)
| Kat.no. | Mofuta oa acid ea nyutlelie | Mofuta oa sampole o sebetsang |
| 4992285 | DNA / RNA | Serum, lero la mali, mokelikeli oa 'mele, lisele, tharollo ea poloko ea swab, seaplane sa setso sa vaerase, jj |
| 4992286 | RNA | Serum, lero la mali, mokelikeli oa 'mele, lisele, tharollo ea poloko ea swab, seaplane sa setso sa vaerase, jj |
| 4992287 | DNA | Libaktheria, li-fungus, likokoana-hloko le likokoana-hloko tse arohaneng le serum, lero la mali, lisele, pleural le ascites, mokelikeli oa cerebrospinal, sekhohlela, bronchoalveolar lavage fluid le parafini |
● TIANSeq DirectFast Library Kit (illumina) (4992259/4992260)
Ho arohana ha Enzymatic DNA. E loketse moaho oa laeborari ea DNA bakeng sa sethala sa Illumina se phahameng sa tatellano
● TIANSeq Fast DNA Library Kit (illumina) (4992261/4992262)
E loketse moaho oa laeborari ea DNA bakeng sa sethala sa Illumina se phahameng sa tatellano
● Fast Kit Library Library bakeng sa Ion Torrent Platform (Customized kit)
E loketse moaho oa laeborari ea DNA bakeng sa sethala sa Ion torrent se phahameng sa tatellano
● Fast Kit Library Library bakeng sa MGI Platform (Customized kit)
E loketse moaho oa laeborari ea DNA bakeng sa sethala sa MGI se phahameng sa tatellano
● TIANSeq rRNA Depletion Kit (H / M / R) (4992363/4992364/4992391) (bakeng sa sethala sa Illumina / Ion torrent / MGI)
Bakeng sa ho tlosoa ha rRNA ea moamoheli e le ho ntlafatsa kutloisiso ea tlhahlobo ea RNA ea vaerase
● TIANSeq Fast RNA Library Prep Kit (Illumina) (4992375)
Bakeng sa kaho ea laebrari ea RNA ea vaerase kamora ho tlosa rRNA ea moamoheli, e ka tsebang kapele ho ba teng ha tatellano ea vaerase ea RNA
● TIANSeq Stranded RNA-Seq Kit (Illumina) (4993007)
Bakeng sa kaho ea laebrari ea RNA ea vaerase kamora ho tlosa rRNA ea moamoheli, e ka khethollang ka nepo phapang ea vaerase
● RNA RNA Library Prep Kit bakeng sa MGI Platform (Customized kit)
E loketse moaho oa laebrari ea RNA bakeng sa sethala sa MGI se phahameng sa tatellano
● TIANSeq Boima ba Khetho ea Lihlahisoa tsa DNA (4992358/4992359/4992979)
Bakeng sa tlhoekiso le khetho ea boholo ba likhechana tsa DNA nakong ea kaho ea laeborari ea DNA
● TIANSeq RNA Lihlahisoa tse Hloekileng (4992360/4992362/4992867)
Bakeng sa tlhoekiso ea RNA kamora ho ruisa RNA
● TIANSeq Adapter-Index Adapter (Illumina) (4992642/4992378)
● Adapter ea TIANSeq Dual-Index (Illumina) (NG216-T1 / 2/3/4/5/6)
Bakeng sa kaho ea laeborari ea DNA bakeng sa sethala se tsamaisang thepa e phahameng sa Illumina
