FastKing One Step RT-qPCR kit

Mohato o le mong oa Real-Time RT-PCR e nang le SYBR-Green.

FastKing One Step RT-qPCR Kit e etselitsoe Real-Time One Step RT-qPCR ka ho sebelisa SYBR Green I. Kit e ka sebelisoa mohatong oa nako ea sebele ea RT-qPCR ea liphofu tsa RNA, ha phetisetso ea morao-rao le PCR li etsahala ka tatellano ka har'a tube e le 'ngoe. Ka hona ts'ebetso e bonolo, mme e fokotsa menyetla ea ho silafala.

Motsoako oa Enzyme ea 25 × RT-PCR ka kiteng ena ke mohlala oa `` TIANGEN's novel reverse transcriptase '' (FastKing RTase), anti-anti-modified hotstart Setso DNA polymerase le RNase Inhibitor. FastKing RTase ke mofuta o mocha oa limolek'hule tse fetotsoeng reverse transcriptase. Ka mochini o kenyellelitsoeng ka ho khetheha oa hydrophobic, o nang le kamano e matla ea RNA le botsitso ba mocheso. FastKing RTase e ntlafalitse ts'ebetso e nepahetseng ea phetisetso le bokhoni ba ho holisa template ea RNA ka sebopeho se rarahaneng sa bobeli. Mofuta o mocha oa hotstartSetso DNA polymerase e nang le ts'ebetso e ntle haholo e sebelisoa ts'ebetsong ea PCR, e etsa hore karabelo ea PCR kamora phetolelo e fetolelang e be le katleho e phahameng le ho khetheha. Ntle le moo, 2 × FastKing RT-qPCR Buffer (SYBR Green) sehlahisoa sena ke sistimi e ncha e arabelang ka ho khetheha e ntlafalitsoeng bakeng sa li-enzyme tse peli tse kaholimo, ho kenyeletsoa likarolo tsa ionic tse hlokahalang, dNTPs, dae ea SYBR Green, bohato bo le bong ba botsitso le bo ntlafatsang. e ka netefatsa hore FastKing RTase le hotstart e nchaSetso DNA polymerase e ka bapala karolo ea bohlokoa ka ho fetesisa mohatong oohle oa mohato o le mong oa ho arabela.

Setsi sena se fana ka li-curve tse tloaelehileng maemong a fapaneng. E ka fumana ka nepo le ka bongata liphatsa tsa lefutso tse nang le bongata bo phahameng le bo tlase ka ho pheta-pheta hantle le ho ts'epahala ho phahameng.