EndoFree Mini Plasmid Kit II

Bakeng sa tlhoekiso ea grade ea plasmid e se nang phetoho ea endotoxin.

EndoFree Mini Plasmid Kit II e sebelisa theknoloji e ikhethang ea silica membrane adsorption ho sebetsa ka nepo le ka kotloloho ho tlama plasmid DNA. Sistimi e ikhethang ea ho tlosa li-endotoxin e ka tlosa litšila ka nepo joalo ka endotoxin le liprotheine. Ts'ebetso eohle ea ho ntša metsi e nka hora e le 'ngoe feela, e leng bonolo ebile e potlakile. Sehlahisoa sa plasmid extraction se amana le palo ea plasmid, mofuta oa libaktheria tse amohelang le maemo a setso. Ho fihlela 70 μg ea plasmid DNA e ka ntšoa ho 5-15 ml ea sehlahisoa sa setso sa libaktheria bosiu bo le bong.

Katse. Che Paka Size
4992422 50 li-preps

Product Qaqileng

Mohlala oa Teko

Lihlahisoa tsa Product

Likaroloana

■ chai e potlakileng le e phahameng: 5-70 μg plasmid DNA e ka ntšoa ka nako ea hora e le 'ngoe.
■ Bohloeki bo phahameng: Sistimi e ikhethang ea ho tlosa li-endotoxin ha e kopantsoe le Spin Column CP4 e ka hlahisa adsorb ea nucleic ka ho khetheha mme ea tlosa endotoxin boemong bo fokolang.
■ Mefuta e mengata ea lits'ebetso: E loketse liteko tsa ka mehla tse kang thibelo ea endonuclease digestion, PCR, tatellano, ligation, phetoho, hammoho le liteko tse nepahetseng haholo joalo ka kalafo ea liphatsa tsa lefutso, microinjection ea sele, ho khutsisa ha liphatsa tsa lefutso, sengoloa, jj.

Tlhahiso ea Plasmid

Plasmid Yield

Lihlahisoa tsohle li ka fetoloa bakeng sa ODM / OEM. Bakeng sa lintlha,ka kopo tlanya Customized Service (ODM / OEM)


  • E fetileng:
  • E 'ngoe:

  • product_certificate04 product_certificate01 product_certificate03 product_certificate02
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    Experimental Example E loketse ho ntšoa ha li-plasmids tse phahameng / tse tlase
    Setšoantšo sa 1: Ho ntšoa ha plasmid DNA ho tsoa mefuteng e fapaneng ea litloaelo tsa libaktheria ho sebelisoa EndoFree Mini Plasmid Kit II.
    Elution bophahamo ba modumo: 200 μl; Ho kenya bophahamo ba modumo: 3 μl plasmid kopi e tlase pBR322, 2 μl kopi e phahameng ea plasmid pBS.
    Electrophoresis e entsoe ka 6 V / cm bakeng sa 30 min ho 1% agarose.
    Ho fetisoa ha sele ka plasmid e hloekisitsoeng
    Experimental Example Setšoantšo sa 2 PEGFP plasmid e hloekisitsoeng ke EndoFree Mini Plasmid Kit II e fetiselitsoe liseleng tsa 293T. Tlhaloso ea GFP e fumanoe ka 48 h kamora ho fetisoa
    Ngola molaetsa oa hau mona ebe u o romella ho rona