■ chai e potlakileng le e phahameng: 5-70 μg plasmid DNA e ka ntšoa ka nako ea hora e le 'ngoe.
■ Bohloeki bo phahameng: Sistimi e ikhethang ea ho tlosa li-endotoxin ha e kopantsoe le Spin Column CP4 e ka hlahisa adsorb ea nucleic ka ho khetheha mme ea tlosa endotoxin boemong bo fokolang.
■ Mefuta e mengata ea lits'ebetso: E loketse liteko tsa ka mehla tse kang thibelo ea endonuclease digestion, PCR, tatellano, ligation, phetoho, hammoho le liteko tse nepahetseng haholo joalo ka kalafo ea liphatsa tsa lefutso, microinjection ea sele, ho khutsisa ha liphatsa tsa lefutso, sengoloa, jj.
Lihlahisoa tsohle li ka fetoloa bakeng sa ODM / OEM. Bakeng sa lintlha,ka kopo tlanya Customized Service (ODM / OEM)
E loketse ho ntšoa ha li-plasmids tse phahameng / tse tlase Setšoantšo sa 1: Ho ntšoa ha plasmid DNA ho tsoa mefuteng e fapaneng ea litloaelo tsa libaktheria ho sebelisoa EndoFree Mini Plasmid Kit II. Elution bophahamo ba modumo: 200 μl; Ho kenya bophahamo ba modumo: 3 μl plasmid kopi e tlase pBR322, 2 μl kopi e phahameng ea plasmid pBS. Electrophoresis e entsoe ka 6 V / cm bakeng sa 30 min ho 1% agarose. Ho fetisoa ha sele ka plasmid e hloekisitsoeng |
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Setšoantšo sa 2 PEGFP plasmid e hloekisitsoeng ke EndoFree Mini Plasmid Kit II e fetiselitsoe liseleng tsa 293T. Tlhaloso ea GFP e fumanoe ka 48 h kamora ho fetisoa |
Ho tloha ha e theoa, fektheri ea rona e ntse e nts'etsapele lihlahisoa tsa maemo a pele ka ho latela molao-motheo
ea boleng pele. Lihlahisoa tsa rona li fumane botumo bo botle indastering ebile li na le boleng bo phahameng har'a bareki ba bacha le ba khale ..