Kit ea EndoFree Midi Plasmid

Bakeng sa tlhoekiso e potlakileng ea li-plasmid DNA ea endotoxin ntle le tšoaetso.

EndoFree Midi Plasmid Kit e sebelisa mokhoa o ikhethileng oa silica membrane adsorption ho sebetsa ka nepo le ka kotloloho ho tlama plasmid DNA. Buffer EBT e khethehileng le Filtration CS1 li ka tlosa litšila ka nepo joalo ka endotoxin le protheine. Ts'ebetso eohle ea ho ntša metsi e nka hora e le 'ngoe feela, e leng bonolo ebile e potlakile.

Katse. Che	Paka Size
4992853	10 li-preps

Romela ya imeile ho re

Product Qaqileng

Phallo ea mosebetsi

Mohlala oa Teko

Lihlahisoa tsa Product

Likaroloana

■ E loketse ho ntša li-plasmid ho tloha ho 50-100 ml ea libaktheria moetlo.
■ Plasmid e hloekisitsoeng e na le "endotoxin" e tlase haholo 'me e ka fihlela litlhoko tsa phetisetso ea boleng bo holimo.
■ Ho bonolo ho sebetsa ka mokhoa o ikhethileng le o potlakileng oa ho tlosa li-endotoxin.

Likopo

Plasmid DNA e hloekisitsoeng e ka sebelisoa litekong tsa baeloji ea limolek'hule le liteko tsa biology ea sele tse kang thibelo ea endonuclease digestion, tatellano, phetoho, phetisetso, microinjection, jj.

Tlhahiso ea Plasmid

Lihlahisoa tsohle li ka fetoloa bakeng sa ODM / OEM. Bakeng sa lintlha,ka kopo tlanya Customized Service (ODM / OEM)

E fetileng: TIANprep Ka potlako Mini Plasmid Kit

E 'ngoe: TIANprep ka potlako N96 Plasmid Kit

	Plasmid pEGFP-C1 e hloekisoa ke EndoFree Midi Plasmid Kit ho tsoa ho 50 ml bosiu bo le bong ba libaktheria. Khakanyo ea plasmid e lekantsoe ke Nanodrop mme chai e baliloe. Sephetho se bonts'a hore chai ea plasmid ea EndoFree Mini Plasmid Kit e phahame ho feta eo ka sehlahisoa se loketseng se tsoang ho Morekisi A.
	Plasmid pEGFP-C1 e ntšitsoeng ke EndoFree Midi Plasmid Kit e fetisetsoa liseleng tsa 80% confluence 293T ke TIANGEN TIANfect. Tlhaloso ea GFP e fumanoe 24-36 h kamora phetisetso. Sephetho se bonts'a hore ts'ebetso ea phetisetso e ne e le 80% -90%.